| Description | 3-D Life FG-Dextran is a component of the 3-D Life Hydrogel system. Itcontains maleimide-functionalized dextran (Mal-Dextran) that can be usedtogether with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat. No. L60-1or L60-3) to set up fast gelling, cell-compatible hydrogels. Dextran-based 3-D Life hydrogels can be dissolved by the addition ofdextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recoveryof chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or forfurther cultivation. 10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gelformation in the presence of cells.For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide. | ||||||||||||||||||||
| Application | 3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution. | ||||||||||||||||||||
| Quantity | Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link). | ||||||||||||||||||||
| Components |
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| Detailed Product Information |
| Fast-gelling Hydrogels |
| Cellendes user guide |
| Degradation of dextran-based hydrogels with dextranase. |
Cellendes
Cellendes为德国Tübingen大学NMI自然科学和医学研究所(NMI Natural and Medical Sciences Institute)的衍生(spinoff)公司,由Brigitte Angres和Helmut Wurst博士与2009年筹建。Cellendes专门为3D细胞培养设计精巧、可操控的培养方案,其产品在基础研究、药物研发和生物医药工程等领域被广泛选用。
Cellendes 3-D Life为3D细胞培养专门设计的整合培养系统。这种水凝胶系统由两种聚合物组成,通过两种聚合物在室温下共价结合交联生成水凝胶。这种化学反应速度快且对细胞无副作用。生成方式操作极其简单,无需额外装置,还可以根据自己的需要在交联前添加生物活性分子,让3D培养更能满足多变的个性化培育需要。
Cellendes是一家生命科学公司,为3D细胞培养中细胞环境的受控设计提供全面的解决方案。试剂工具箱可在用户工作台上灵活地组成仿生水凝胶。应用领域包括基础研究,药物开发和生物医学工程。